1 / 1
|
DeCS
|
|
|
Descriptor English:
|
|
Chromatin Immunoprecipitation
|
Descriptor Spanish:
|
|
Inmunoprecipitación de Cromatina
|
Descriptor Portuguese:
|
|
Imunoprecipitação da Cromatina
|
Synonyms English:
|
|
Immunoprecipitation, Chromatin
|
Tree Number:
|
|
E05.393.170
E05.478.605.160
|
Definition English:
|
|
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. |
History Note English:
|
|
2005
|
Allowable Qualifiers English:
|
|
|
Record Number:
|
|
38826
|
Unique Identifier:
|
|
D047369
|
Occurrence in VHL:
|
|
|
Similar:
|
|
DeCS CID-10 SciELO LILACS LIS
|